Molecular identification procedures generally include the analysis of proteins by electrophoretic techniques, such as sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing (IEF), capillary electrophoresis (CE) or immunoassay techniques (Huang et al., 2008). In some cases morphological features are of limited value for identification and differentiation purposes, even with whole specimens, because they can show either considerable intraspecific variations or small differences between species (Teletchea, 2009 Chen et al., 2012). Oocytes were ovulated in the spawning period and Were observed from March to November but it was prominent in May. Matured oocytes occupied the most areas of the ovary which Perinucleolus stage was recorded in the month of January. Percentage of oogonia in the ovary recorded in the month of November and highest percentage of early and late The mature testis began to develop in the early April and was prominent from May to July. March contained mostly spermatogonia, spermatocytes and spermatids, although a small amount of spermatozoa wasĪlso found in March.
The testis at early development stages between October and
Oocytes and maturation stage) and atretic stage were differentiated in ovary. Perinucleolus stage and late early perinucleolus stage), vitellogenesis (early vitellogenic oocytes, advanced vitellogenic Three developmental stages of oocytes such as, ogenesis (oogonia, early Four developmental stages such as, spermatogonia, spermatocytes, spermatidsĪnd spermatozoa were identified in testis. Developmental stages of both male and female gonad and estimation of gonadosomatic index (GSI) of Monopterus cuchia
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